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KMID : 0545120180280040566
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 4 p.566 ~ p.570
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Synthesis of Aesculetin and Aesculin Glycosides Using Engineered Escherichia coli Expressing Neisseria polysaccharea Amylosucrase
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Park So-Yoon
Moon Keum-Ok
Park Cheon-Seok
Jung Dong-Hyun
Cha Jae-Ho
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Abstract
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Because glycosylation of aesculetin and its 6-glucoside, aesculin, enhances their biological activities and physicochemical properties, whole-cell biotransformation and enzymatic synthesis methodologies using Neisseria polysaccharea amylosucrase were compared to determine the optimal production method for glycoside derivatives. High-performance liquid chromatography analysis of reaction products revealed two glycosylated products (AGG1 and AGG2) when aesculin was used as an acceptor, and three products (AG1, AG2, and AG3) when using aesculetin. The whole-cell biotransformation production yields of the major transfer products for each acceptor (AGG1 and AG1) were 85% and 25%, respectively, compared with 68% and 14% for enzymatic synthesis. These results indicate that whole-cell biotransformation is more efficient than enzymatic synthesis for the production of glycoside derivatives.
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KEYWORD
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Aesculetin, aesculin, amylosucrase, biotransformation, Neisseria polysaccharea, transglycosylation
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